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PROCEDURES FOR panLFRAPID |
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BENEFITS
• Results in 15 minutes
• No special equipment is
needed
• Results are easy to interpret
• Blood, serum or plasma can
be used as sample
• Sample collected any time
of the day can be used
• No sample preparation
• Small volume (25 µl) of
sample used
• 95% sensitivity
• 100% Specificity |
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i Add serum / plasma / whole blood.
For serum / plasma sample:
Add 25 µl sample to square well. Sample will start wicking up the membrane. Wait till the wet sample front reaches the blue line.
For whole blood sample:
Add 35 µl sample to square well followed by 1 drop of chase buffer to the same well. Sample will start wicking up the membrane. Wait till the wet sample front reaches the blue line. |
PERFORMANCE CHARACTERISTICS
Method : Immunochromatography
Detection : Human IgG4 against a specific B. malayi and W. bancrofti recombinant proteins
Specimen : Serum, plasma and whole blood
Specimen volume : 25 µl (serum/plasma); 35 µl
(whole blood)
Shelf life : 18 months
Assay time : 15 minutes |
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ii Add 2 drops of chase buffer to well 2.
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APPLICATION
For detection of individuals with bancroftian and/or brugian filariasis including mapping of population in areas with the disease.
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iii
Pull clear tab until resistance is felt.
Add 1 drop of chase buffer to square well.
Start timing 15 minutes. |
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INTERPRETATION OF RESULTS
1. Positive for either or/and Wuchereria brancrofti, Brugia malayi and Brugia timori specific antibodies if coloured bands appear at the Control line (A) and Test line(s) at B, C or both
B & C as shown in the diagram above. Any intensity of band should be considered as positive.
2. Negative for Wuchereria brancrofti, Brugia malayi and Brugia timori specific antibodies if only the Control line (A) is visible through the viewing window.
3. Invalid if the Control line (A) is absent. If this occurs, the assay should be repeated using a new test cassette. |
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